Tissue culture or Molecular-work?

mana lebih penat?? antara dua ni.. aku rasa buat tissue culture sesungguhnya lebih penat penat penat dari molecular work... dah laa penat.. pastu kalau contaminate.. browning... aduhaiiii~ letih nak revive cell yg cantik!!~ kalau molecular work, ermm challenges dia sbb benda tu kt tak nampak with aked eyes.. if buat keje tak focus, mesti tak sure da mskkn atau belum in the reaction mixture.. but one thing.. kalau nak repeat, tak de laa lama sgt amik masa.. contoh.. buat pcr.. pastu view gel.. kalau tak de band hari ni, esok boleh repeat balik.. tak amik masa yg lama pun... antara rajin atau tak je.. tissue culture plak on the other hand.. kena rajin and luck.. subculture on time.. kalo tak cell tu mampus, lagi pening kepala.. then.. nak prepare barang lagi satu hal.. media.. pastu, lepas subculture, nak basuh glassware segala tok nenek lagi.. huh~ keje yg paling aku tak suka.. tu laa dia.. basuh glassware berbesen-besen.. penat wooo... nak kasi bersih betul2.. kalo contam kena autoclave dulu.. pastu kasi rinse ngan dH2O lagi.. mmg PENAT.... kalau aku balik lambat sbb buat tissue culture, aku nye energy mmg low abis.. ibarat ultraman dah habis power.. hati dia dah kasi warning.. cam tu laa.. mata plak dah blur2 sket.. huhu~ kepala sure berdenyut2.. keje molecular.. kalau aku balik lewat, aku steady lagi.. hehe.. mcm hari ni.. anyway, banyak benda yg aku belajar dari kedua-dua field ni.. sometimes, ada skill2 kat molecular yg applicable in tissue culture.. ada gak sebaliknya.. sekarang ni aku dah start molecular work.. so, kena bahagi masa buat 2 benda dlm 1 week.. pastu analyse transcriptome data... bila2 ada masa senggang atau buat malam (mampu kah???).. tak kisah laa cam mana tunggang terbalik hidup aku lepas ni.. keje mesti kena jalan!!!~ cahaya di hujung tunnel dah nampak dah.. cuma kena usaha ke arah cahaya itu.. semoga aku berjaya... semoga Allah sentiasa mempermudahkan aku...

Manuscript

Aku dah rasa cam nak muntah ngadap laptop ni prepare manuscript.. total word counts skrg ni dlm 9,000... sikit lagi nak siap!!~ go!! go!!~ huh~ penat gila siapkan benda ni.. yg paling tak best tu... aku misplaced data.. hoho~ complicated betul.. kena check balik kat mesen HPLC.. tu 1st part.. yg 2nd part punya hal lagi laa.. huhuh masih ada 400+ unigenes utk aku analyse.. adakah dia up- or down-regulate.. even ada excell yg boleh tolong aku sort the data.. but still.. i need to copy + paste it manually satu2.. sbb aku tak reti pakai perl/linux script (kalau wujud) utk extract out semua unigene yg up- or down- kat specific pathways.. Makan minum dah tak terurus.. tdo pun tunggang terbalik!!~ apa2 pun yg terjadi.. aku tetap kena dtg 1 week workshop on bioinfo tu!! AND NOT TO FALL SLEEP during lecture~ haha... so far, so good.. aku tak ngantuk pun.. alhamdulillah... but that workshop mmg bagus utk aku pun... ok!!~ setelah tdo selama sejam kejap td.. aku skrg sudah semangat semula utk meneruskan write-up ni.. go!!~ go!!!~

Candidature Defense...

This is my 5th Semester in UM.. next month will be my candidature defense for my phd candidature.. lalala.. i'm so exhausted preparing the manuscript for this CD.. The deadline to submit is NEXT WEEK FRIDAY!!~ 4 copies + 10,000 words... The hardest part was gathering the data and also to analyze some of bioinfo data that I got end of year 2010.. I was totally headache!!!~ I'm cracking my head on thinking how I gonna present those data + the analyzing data part is still ongoing, not completely done yet.. I'm running out of time!!!!!~ The best part is.... I got ONE week workshop next week starting on Monday to LOVELY Friday (deadline CD submission).. So, guys, please pray for me.. Hopefully this manuscript things done by tomorrow, so I can ask my bos to do some corrections b4 submission... lalalala~ good luck to me, ya.... Oooohh yeah.. i'm absorbing all lucks in this universe...

"Ya Allh, permudahkan lah segala urusan cik purplepink"