keje mcm bukit...

bila aku terlalu banyak sgt keje yg nak kena settle at one time, kdg2 tiba mood tak tentu arah ni, aku jd reluctant utk buat satu keje pun.. huhuhu~ terus jd manusia paling tak guna satu hari tu.. ampeh laa.. aku cam kene berperang dgn diri sendiri... kejadian mcm ni selalu melanda aku time weekend je.. time weekdays tak laa sbb aku takut pecah amanah time keje... walaupun aku tak secara officialnya keje.. aku student, tp aku ada responsibility utk siapkan research aku tu.. tak de laa aku mengular.. takut2 duit aku dpt tak berkat...

ermm tp kdg2 tu, laju je plak siapkan keje.. keje tak berhenti.... mood mcm ni kalau dtg, aku mmg happy laa walaupun aku penat.. sbb at least aku tau aku penat sbb buat keje.. keje pun jln... sampai msk dlm mimpi2 aku.. huhu~ cam workaholic gila la kan... ermm aku ni mmg tak balance betul~ 

aku kena atasi tak de mood yg sekali sekala dtg tu laaa... ermm ke sbb aku dah lama tak balik JB ni??? sbb rindu kat mak, abah, adik2.. dan makanan fav aku.. may b kot.. nak balik laa cam ni.. aku dah lama tak balik.. aku ni mmg manja sikit... tp mak abah aku tak kisah pun aku manja2 ngan diorg walaupun dah besar panjang... unconditional love~ ye laa hidup ni kan sekali je.. kita tak boleh nak ulang hari2 yg berlalu.. oleh itu, baik laa kita appreciate apa yg kita ada sekarang ni.. masa ini... masa single2 ni.. (aku da start merapu, sorry)..

kat lab, aku ada 2 org sumber inspirasi... sorg kat lab pbiu, sorg kat lab bgm1... sumber inspirasi aku ni dua2 kakak lab aku yg dah kawen dan ada anak.. aku suka laa tgk semangat diorg nak siapkan research diorg!!~ sgt determine!!~ tp tu laa kdg2 mmg laa ada down period.. tp mereka pandai mengawal emosi.. tak mcm aku.. aku kan budak.. hahaha.. mmg lots more to learn laa from them... kalau aku penat buat keje, pastu tgk diorg, mesti terdetik aku kata dlm hati.. 'diorg lagi penat... ni sikit je ni percentage penat aku kalau nak banding dgn diorg'.. so, aku pun endure penat aku tu dan sambung buat keje.. (ni time mood buat keje laju) haha.. 

well... aku ada jalur takdir aku sendiri dlm hidup ni.. may b jalur takdir aku ni kata aku mmg org yg paling sesuai utk siapkan research yg aku tgh buat ni.. aku paling competent.. paling sabar.. paling segala-galanya.. cuma aku yg tak sedar kot.... oleh itu, aku kena buat sehabis baik (cam lagu pengakap) hahahaha.... bila aku kata kat mak aku, aku kdg2 rasa malas especially time weekend, mak aku kata, tak pe... mak aku lagi suka aku cam ni drpd buat keje 24-7... sbb kita kan human.. i'm her human daughter.. haha.. apa aku merepek, daaa... dia risau aku tak rest.. dia kata laa... tp post-doc yg supervise aku plak kata... bdk phd mmg tak de rest.. kene keje je 24-7.. huh~ sebagai manusia biasa, aku tak mampu, kot.. sbb aku ada time2 malas aku..... cuma aku harap, bila time rajin aku tiba.. dpt counter back progress yg tak achieve time malas tersebut...

sejak dua menjak ni.. (actually sbb broadband aku da laju), rajin plak aku drop post kat bloggu ni.. oohh semestinya laa sbb aku rasa aku dok merapu sensorg je.. tak de org baca, kot... hahahhaha~ good luck to me, ya!!~ tomorrow will be my candidature defense.. takut gak sebenarnya...

bila bos dah mengamuk

td ada meeting.. huhu~ bila sorg bos naik angin... bos2 yg lain pun sama2 naik angin.. huhu~ mmg laaa kena marah kaw-kaw punya.. hahaha... dah tak de ampun... ya.. all of my big bosses are super-busy women... i admire them!!~ really... diorg stress tu.. ye laa ada banyak responsibilities lain... so, tadah je laa telinga bila kena marah.. bagus sebenarnya marah... tu tanda sayang.. tanda kita buat salah.. yg baik jadikan tauladan.. yg buruk jadikan sempadan... ermm sebenarnya mereka marah pd org lain dlm group.. tp tempiasnya terkena juga kat semua org... huhu~ nak buat cam ne.. kalau tak, tak de lah pepatah "kerana nila setitik, rosak susu sebelanga"... aku modified sikit jd pepatah scientific nya... "kerana kuman satu cell, rosak callus satu bioreactor".. ni aku punya version =) ... kalau kak azma dgr ni, sure dia gelak.. hahahahaha~~ kalau korg nak tau, kat bawah ni salah satu contoh bioreactor.. ada mcm2 jenis sebenarnya.. yg ni diorg culture ginseng.. kat lab aku, culture callus.. lain laa sikit rupa dia.. tp basicly utk propagate cell jd banyak dgn kadar yg cepat...
sumber gambar ada kat bawah ni, in case korg nak tau lebih detail... huhu~

http://www.springerimages.com/Images/RSS/1-10.1007_s11738-008-0181-3-1

Technology

Cerita telur: 

Dua tiga hari ni, Malaysia kesayangan dah digemparkan oleh berita fake eggs from China.. aku rasa fake eggs ni dah lama kot wujud kat pasaran, tp kita je yg tak tau.. dunia... dunia... telur pun ada yg palsu.. but amazingly, pada aku org China ni bijak... ermm may be juga sbb mereka terdesak sgt sbb nak keluar dari kemiskinan, lalu mereka sanggup menipu org awam dgn menjual fake eggs yg untungnya berlipat kali ganda.. didorong pula dgn tak de pegangan agama yg lurus dlm jiwa dan etika yg baik.. walaupun manusia berjaya mencipta telur ayam yg hampir sama dgn ayam, Maha suci Allah.... telur palsu masih tak dpt menandingi telur asli yg dihasilkan oleh si ayam.. telur asli ada identity yg tersendiri... telur asli ada selaput telur dan ruang udara!!!~ so, kalau korg nak masak telur, check laa dulu.. kot2 kena tipu.. huhu~ seperti mana yg kita semua tahu, telur merupakan sumber protein yg murah.. telur palsu tak dpt menandingi quality protein yg ada pd telur asli sbb mainly diperbuat dari bahan kimia (ada org claim that they use chemicals yg merbahaya utk kesihatan). tp aku tak tau laa kesahihannye.. sbb they claimed that benzoic acid (salah satu bahan membuat telur putih dan kuning) and calcium chloride (salah satu bahan dlm kulit telur) adalah merbahaya.. tp aku rasa makann lain pun ada benzoic acid tu.. sbb kan benzoic acid tu food preservative? ermm tak reti laa nak komen.. ilmu aku ada batasannya. mungkin aku yg silap.. ikut logic akal, kenapa laa nak buat telur ayam palsu ni?? bukan nye mahal sgt telur ayam yg ori, kan?? bak kata salah sorg labmate aku... tu kata kita kat Malaysia.. tp may b kata org kat China, telur ayam yg ori pun mahal.. huhu~ sbb tu ada alternative lain...

Cerita Sequencing:

Smlm aku ada seminar kat Malaysian Genome Institute (MGI) kat Bangi.. bangunan baru!!~ cantik, besar.. flooring dia fuuhhh!!~ boleh buat cermin!!~ serious aku tak tipu!!!~ berkilat.... tp rasa mcm kosong je.. actually bangunan tu belum launching pun lagi.. baru siap.. pekerja pun tak ramai lagi. so, aku cam ada cita2 utk apply keje kat sini je.. hehehehehe.... ok, what!!~ tgk laa rezeki kat mana lepas grad nanti.. aku mmg adalah manusia yg tak de plan kehidupan!!!~ tgk laa rezeki mana yg dtg dulu... ermm sbb ada ura2 may b sambung buat research project aku kat UM lepas aku grad. aku tak kisah pun.. ok.. mari cerita pasal MGI.. mmg best kot keje kat sini.. siap ada special room utk PCR, incubation dan sebagainya (aku tak sempat nak tour satu bangunan).. dia punya washroom mcm ala2 kat shopping complex, gitu... class~ ermm tp... aku nampak ada satu je high-end sequencer.. Illumina-Solexa sequencer.. mcm sedih je.. hopefully the number will increase in the future.. Sequencing technology skrg dah jadi cutting-edge technology in this era.. routine work.. tambahan pula nama besar mcm MGI.. so, i think... sequencer tu kena laa banyak lagi kalau kita nak compete dgn negara2 lain... As lawatan aku p Beijing Genome Institute (BGI), Shenzen, China last year... they have 32 high-end sequencer!!~ imagine!!!~ and the number will increase for sure!!~ aku rasa kagum!!!~ mmg ada satu bilik khas letak sequencer sahaja.. berderet memanjang!!!~ tp tak boleh amik gambar.. huhu~ China mmg dah lama tinggalkan kita dlm bab2 sequencing tehcnology ni.. they even thought about the third-generation of sequencer!!~ to sequence only selected part or selected cells!!~ or even one cell from specific part of organism!!~ wow!!!!!!~ yeah.. it really2 a brilliant approach especially in medical world, as you can compare healthy cells with cancer cells for instance.... and the server kat sana..  mmg power!!~ bertingkat-tingkat!!!~ aku tak tau laa Malaysia mampu sampai tahap mcm tu ke tak.. ermm high-end sequencer mmg mahal.. kita ada budget ke tak?? tp China government.. they got money to allocate to the technology!!~ siapa kata China miskin??? tak, kan..... mereka kaya sebenarnya... mereka ada perancangan yg strategik... bangunan BGI tu adalah bangunana factory yg diubah suai jd research institute.. ada basement utk car park (mcm ala2 shopping complex - jimat spcae).. human resource... semua org tau... sequencing technology meaning u must have workers/researchers yg ada good brians to analyse the sequence.. to assemble back the short DNA or cDNA fragments... must be strong in bioinformatics... scriptings... algorithms... biology knowledge.... kena ada org2 bijak!!~ kat BGI, rata2 semua researchers mereka adalah org2 muda, umur lingkugan 20-26).. fresh graduates from local China Universities... rata2nya adalah org2 bijak yg dpt scholarship time belajar... mcm mana diorg boleh hire mereka yg bijak2 ni?? rahsianya... mereka yg buat syllabus buku text sekolah menengah... dan diorg collaborate dgn university2 tempatan diorg utk hasilkan ramai org2 bijak ni... cuba bayangkan.. dari sekolah menengah lagi bdk2 tu dah didedahkan dgn knowledge yg mereka perlu tahu utk kerjaya mereka.. diorg dah siap2 ada main stream time high school lagi... sure laa ilmu tu melekat.. to them, jd researcher is pekerjaan yg mulia, tingkatkan taraf hidup.. ok.. so, Malaysia.. especially MGI.. i hope that we can learn from BGI on how they plan upgrading their research technology.. mcm mana nak upgrade research area in Malaysia.. bangunan canggih dah ada.. so, kena hire researcher2 yg berkualiti + allocate budget, ye!!~  hehe....

my sweet weekend...

hehe.. after few weekends struggling.. spending days and nights in the lab.. stay back for few days... this weekend is my sweetest weekend ever!!~ I refused to go to the lab.. just rest and lay back at home.. ALONE... yeah.. because my cousin stay at her friends' place.. I dont really care, pun.. suka ati dia laa.. 

my lepaking series events was initially begins with my not-so-late wakeup saturday morning.. actually yesterday I planed to go to the lab.. but somehow, I feel so lazy.. ermm... end up with staying at home.. watching movie...... and went to mid valley.. enjoying my DOUBLE-scooped baskin robin's ice cream... hehe.. it so relaxing... it feel so good.. especially when the ice cream melt in your mouth.. sweet-sour-coffee flavours.. all bend together in my mouth.... haha.. my fav. flavours.. jamoca almond fudge + rainbow sherbert.. nyummy.. so nyummy.. really really nyummy!!~ actually I craved for BR last friday.. but..................... i went back late.. about 9 pm.. and it was not-so-brilliant idea to go to mid that nite.. (they will close at 10pm)

yesterday, I sat alone enjoy my ice cream at BR's table.. watching this one malay couple.. also enjoying their BR.. but instead of at least one scoop/person, they share ONE scoop for two of them!!~ can u imagine!!~ ooohh i dont know.. how to say.. is it because of jimat?? or kedekut?? or they feel it was romantic sharing the ice cream?? huhu~ dunno... tak puas, kot mkn half-scoop je... (to me laaa) 

then, next fav station.... i went to MPH: the bookstore!!!~ I love the smell of books... especially new one.. I feel so refresh!!!~ so relaxing!!~ ermmm I love reading.. it was my fav hobbies since I was a little girl.. I spot not-so-new Sophie Kinsella's novel.. Mini Shopaholic in paperback edition!!~ wow!!~ I want it!!~ the cover is so cute.. blue with a little girl.. Becky's daughter; Minnie.. and also I saw Cecelia Ahern's new novel.. can t remember the title.. but something to do with "Mirror"... huhu~ but i dont plan to buy those.. so, I just wander around the bookstore... flipping through some random pages... from random novels and random mags.. hahha... I love doing this!!!~ I want to buy new book.. or novel.. but... ermm I dont exactly have much time to read it.. in fact, i got more than 3 books that i havent finish read it yet.. huhu~

after maghrib.. i went to another fav spot... it is called Speedy...... the house of CDs and DVDs... i bought 2 DVDs... "Tangled" and "Konsert Alam - M. Nasir".... Tangled... or Rapunzel.... the story of lost princess with long-magic-hair... I love Maximus - the palace white horse the most!!~ he is really funny!!~ especially his facial expression when fight with Eugene!!!~ hahaha... in general, i like the storyline.. Rapunzel is different from other disney princesses characters..  she is much more adventurous!!~ and artistic!!~ ermm... for the M.Nasir's consert.. i bought it for my father.... My family.. we all big fan of M.Nasir especially my father.. my fav song in this concert was... the top list is... 1- keroncong untuk ana, 2- ada, 3- sentuhan listrik mu, 4- dah lagu tu and 5- langgam pak dogo.. these are my top list.. but, the other songs was awesome too!!~ lagi satu yg aku suka tak de dlm this consert.... srikandi cintaku....

just before i get back home.. i bought myself dinner and some energy bars to put in my lab.. since i always stay back late, and in case i feel hungry.. at least i got something to eat before a proper meals..

today... sunday.... ermmm home alone as usual.. literally do nothing significantly related to my research.. huhu~ watching those new DVDs... re-read my fav novel by Sophie Kinsella.... Twenties girls.. it is a story of unfinished business of a ghost name Sadie.. she haunt her great niece to help her get back her precious necklace.... unrealistic story with great ending.. huhu~

tomorrow is monday... esok kena semangat buat keje sbb dah melepak dgn jayanye this weekend..... hehe..

Tissue culture or Molecular-work?

mana lebih penat?? antara dua ni.. aku rasa buat tissue culture sesungguhnya lebih penat penat penat dari molecular work... dah laa penat.. pastu kalau contaminate.. browning... aduhaiiii~ letih nak revive cell yg cantik!!~ kalau molecular work, ermm challenges dia sbb benda tu kt tak nampak with aked eyes.. if buat keje tak focus, mesti tak sure da mskkn atau belum in the reaction mixture.. but one thing.. kalau nak repeat, tak de laa lama sgt amik masa.. contoh.. buat pcr.. pastu view gel.. kalau tak de band hari ni, esok boleh repeat balik.. tak amik masa yg lama pun... antara rajin atau tak je.. tissue culture plak on the other hand.. kena rajin and luck.. subculture on time.. kalo tak cell tu mampus, lagi pening kepala.. then.. nak prepare barang lagi satu hal.. media.. pastu, lepas subculture, nak basuh glassware segala tok nenek lagi.. huh~ keje yg paling aku tak suka.. tu laa dia.. basuh glassware berbesen-besen.. penat wooo... nak kasi bersih betul2.. kalo contam kena autoclave dulu.. pastu kasi rinse ngan dH2O lagi.. mmg PENAT.... kalau aku balik lambat sbb buat tissue culture, aku nye energy mmg low abis.. ibarat ultraman dah habis power.. hati dia dah kasi warning.. cam tu laa.. mata plak dah blur2 sket.. huhu~ kepala sure berdenyut2.. keje molecular.. kalau aku balik lewat, aku steady lagi.. hehe.. mcm hari ni.. anyway, banyak benda yg aku belajar dari kedua-dua field ni.. sometimes, ada skill2 kat molecular yg applicable in tissue culture.. ada gak sebaliknya.. sekarang ni aku dah start molecular work.. so, kena bahagi masa buat 2 benda dlm 1 week.. pastu analyse transcriptome data... bila2 ada masa senggang atau buat malam (mampu kah???).. tak kisah laa cam mana tunggang terbalik hidup aku lepas ni.. keje mesti kena jalan!!!~ cahaya di hujung tunnel dah nampak dah.. cuma kena usaha ke arah cahaya itu.. semoga aku berjaya... semoga Allah sentiasa mempermudahkan aku...

Manuscript

Aku dah rasa cam nak muntah ngadap laptop ni prepare manuscript.. total word counts skrg ni dlm 9,000... sikit lagi nak siap!!~ go!! go!!~ huh~ penat gila siapkan benda ni.. yg paling tak best tu... aku misplaced data.. hoho~ complicated betul.. kena check balik kat mesen HPLC.. tu 1st part.. yg 2nd part punya hal lagi laa.. huhuh masih ada 400+ unigenes utk aku analyse.. adakah dia up- or down-regulate.. even ada excell yg boleh tolong aku sort the data.. but still.. i need to copy + paste it manually satu2.. sbb aku tak reti pakai perl/linux script (kalau wujud) utk extract out semua unigene yg up- or down- kat specific pathways.. Makan minum dah tak terurus.. tdo pun tunggang terbalik!!~ apa2 pun yg terjadi.. aku tetap kena dtg 1 week workshop on bioinfo tu!! AND NOT TO FALL SLEEP during lecture~ haha... so far, so good.. aku tak ngantuk pun.. alhamdulillah... but that workshop mmg bagus utk aku pun... ok!!~ setelah tdo selama sejam kejap td.. aku skrg sudah semangat semula utk meneruskan write-up ni.. go!!~ go!!!~

Candidature Defense...

This is my 5th Semester in UM.. next month will be my candidature defense for my phd candidature.. lalala.. i'm so exhausted preparing the manuscript for this CD.. The deadline to submit is NEXT WEEK FRIDAY!!~ 4 copies + 10,000 words... The hardest part was gathering the data and also to analyze some of bioinfo data that I got end of year 2010.. I was totally headache!!!~ I'm cracking my head on thinking how I gonna present those data + the analyzing data part is still ongoing, not completely done yet.. I'm running out of time!!!!!~ The best part is.... I got ONE week workshop next week starting on Monday to LOVELY Friday (deadline CD submission).. So, guys, please pray for me.. Hopefully this manuscript things done by tomorrow, so I can ask my bos to do some corrections b4 submission... lalalala~ good luck to me, ya.... Oooohh yeah.. i'm absorbing all lucks in this universe...

"Ya Allh, permudahkan lah segala urusan cik purplepink"